Molecular Identification of Aeromonas veronii Isolated from Soft-shelled Turtles and Phylogenetics Analysis of Aeromonas(Aeromonadaceae)

Aeromonas is a kind of bacteria which could infect human and aquatic animals.One Aeromonas strain of pathogenic bacteria HBJY01,isolated from diseased Trionyx sinensis,was identified through molecular method.Phylogenetics status of Aeromonas(Aeromonadaceae) was analyzed.Using the 16S rRNA and gyrB genes,the Aeromonas strain was identified,bacterial phylogenetics trees were constructed and pairwise distance was calculated.Based on these,phylogenetics status of Aeromonas(Aeromonadaceae) was analyzed.HBJY01 was very similar to Aeromonas veronii in terms of 16S rRNA and gyrB gene sequences.In the genetic distance,Aeromonas and other Vibrionaceae had great differences.HBJY01 was identified as Aeromonas veronii.Aeromonas should be an independent new family,Aeromonadaceae,not be a genus of Vibrionaceae.