Effect of Connexin 43 on Myocardial Protection in Ischemic Preconditioning

Objective To investigate the effect and status of connexin 43 on myocardial protection in ischemic preconditioning.Methods 48 Sprague-Dawley rats were randomly divided into four groups: S,IR,IP,5-HD+IP.The myocardial infarct size and arrhythmia score were measured.The expression of connexin 43 was detected by RT-PCR.Results Compared with group IP,arrhythmia score and IS/AAR was higher and the expression of connexin 43 was notablely lower in group IR and group 5-HD+IP(P0.01).Ischemic preconditioning might reduced arrhythmogenesis and myocardial infarct size,meanwhile enhanced the expression of connexin 43.5-hydroxydecanoate acid might interdicted the myocardial protection of IP(P0.01).Conclusion Ischemic preconditioning might play an important role in myocardial protection by activating mitochondrial ATP sensitive potassium channel and preserving connexin 43 posphorylation.