基因敲除
RNA干扰
生物
转录调控
基因
毒死蜱
细胞生物学
转录因子
核受体
击倒阻力
褐飞虱
黑腹果蝇
基因表达调控
抄写(语言学)
遗传学
基因表达
发起人
酵母
电泳迁移率测定
戒毒(替代医学)
抗药性
核糖核酸
基因沉默
分子生物学
核出口信号
作者
Kai Lin,Zichun Zhong,Yutao Hu,Hongxin Wu,YuJing Guo,Sunbin Huang,Haoming Wu,Xiaoxia Xu,Fengliang Jin,Rui Pang
标识
DOI:10.1021/acs.jafc.5c08828
摘要
Insecticide resistance has led to the failure of chemical pest control and threatens global food security, with transcriptional regulation of detoxification genes by xenobiotic-response transcription factors (TFs) playing a key role. However, the regulation mechanism remains unclear in most crop insect pests. This study characterized the role of the nuclear receptor NlNR2E in response to insecticides in the brown planthopper Nilaparvata lugens, a destructive rice pest. Using DAP-seq, we identified detoxification gene promoters targeted by NlNR2E. NlABCG23, encoding an ATP-binding cassette (ABC) transporter, showed significantly reduced expression after the RNA interference (RNAi) knockdown of NlNR2E. Dual-luciferase reporter, yeast one-hybrid, and electrophoretic mobility shift assays confirmed that NlNR2E directly binds to NlABCG23 promoter, thus activating NlABCG23 expression. RNAi knockdown of NlABCG23 significantly reduced resistance to imidacloprid, dinotefuran, pymetrozine, and chlorpyrifos in N. lugens. These findings illustrated how xenobiotic-induced TF regulates the ABC transporter, conferring resistance to multiple insecticides in planthoppers.
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