シロイヌナズナ汎用ジーンターゲティング技術の開発 : 平成24-26年度成果報告書

月 日現在 ２７ ６ １０円 3,000,000研究成果の概要(英文)：Gene-targeting (GT) is a technology to either disrupt or modify specific gene(s) on the genome, and a power tool to study gene functions in the field of basic research, as well as to improve crop species in application.In higher plants, however, GT technology is not widely used, due to the fact that plants generally have low rate of homologous recombination.In this study, I established a cell culture of reprogrammed Arabidopsis cells to be used as a host for high-efficiency transformation, and constructed GT-vectors for Arabidopsis.After several transformation trials, it was found that the transformation efficiency is not high enough for GT experiments, and that further investigation is necessary to achieve the GT technique.