Swimming training activated adenosine monophosphate-activated protein kinase (AMPK) in AMPKα2<sup>+/+</sup> mouse hearts but not AMPKα2<sup>-/-</sup> mice.

<p>(A) Western blot analysis of protein levels of phosphorylated and total AMPK at different swimming times in AMPKα2^<b>+/+</b> mice. (B) Quantification of phosphorylated AMPK relative to total AMPK (all n = 4). ^<b>*</b><i>P</i><0.05 swim vs. sed 2 weeks after swimming; ^<b>#</b><i>P</i><0.05 swim vs. sed 4 weeks after swimming. (C) Western blot analysis of phosphorylated AMPK, total AMPKα, AMPKα2 and eukaryotic translation initiation factor 5 (eIF5) at the end of swimming training in AMPKα2^<b>+/+</b> and AMPKα2^<b>-/-</b> mice. (D) Quantification of phosphorylated AMPK relative to eIF5 (n = 6). ^<b>***</b><i>P</i><0.001. Data are mean±SEM.</p>