适体
凝血酶
DNA
指数富集配体系统进化
SELEX适体技术
化学
核酸
生物化学
寡核苷酸
碱基对
分子生物学
生物
重组DNA
生物物理学
小分子
核糖核酸
结合位点
DNA连接酶
基因
血小板
免疫学
作者
Louis C. Bock,Linda L. Griffin,John Latham,Eric Vermaas,John M. Toole
出处
期刊:Nature
[Springer Nature]
日期:1992-02-06
卷期号:355 (6360): 564-566
被引量:2107
摘要
Aptamers are double-stranded DNA or single-stranded RNA molecules that bind specific molecular targets. Large randomly generated populations can be enriched in aptamers by in vitro selection and polymerase chain reaction. But so far single-stranded DNA has not been investigated for aptamer properties, nor has a target protein been considered that does not interact physiologically with nucleic acid. Here we describe the isolation of single-stranded DNA aptamers to the protease thrombin of the blood coagulation cascade and report binding affinities in the range 25-200 nM. Sequence data from 32 thrombin aptamers, selected from a pool of DNA containing 60 nucleotides of random sequence, displayed a highly conserved 14-17-base region. Several of these aptamers at nanomolar concentrations inhibited thrombin-catalysed fibrin-clot formation in vitro using either purified fibrinogen or human plasma.
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