生物
荧光
钙
细胞内
生物学中的钙
细胞生物学
生物物理学
内科学
光学
医学
物理
作者
Joseph P. Y. Kao,Li Gong,Darryl A. Auston
标识
DOI:10.1016/b978-0-12-374841-6.00005-0
摘要
The use of fluorescent indicators for monitoring calcium (Ca2+) signals and for measuring Ca2+ concentration ([Ca2+]) in living cells is described. The following topics are covered in detail: (1) ratiometric and nonratiometric fluorescent indicators and the principles underlying their use, (2) techniques for loading Ca2+ indicators and Ca2+ buffers into living cells, (3) calibration of indicator fluorescence intensity measurements to yield values of intracellular [Ca2+], (4) analysis of nonratiometric fluorescence intensity data and caveats relating to their interpretation, (5) techniques for manipulating intracellular and extracellular [Ca2+], and (6) the use of fluorescent indicators to monitor Ca2+ signals in mitochondria. The chapter aims to present these fundamental topics in a manner that is practically useful and intuitively accessible. The origins of key mathematical equations used in the article are outlined in two appendices.
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