基因亚型
斑马鱼
生物
分子生物学
信使核糖核酸
基因
逆转录酶
实时聚合酶链反应
逆转录聚合酶链式反应
基因表达
计算生物学
聚合酶链反应
遗传学
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 25-46
标识
DOI:10.1007/978-1-0716-2217-9_2
摘要
Reverse transcription quantitative PCR (RT-qPCR) to quantify gene expression is a key molecular technique for the detection of mRNAs due to amplification of transcripts that may be present in low abundance. The technique also permits detection of mRNAs for different isoforms of proteins due to the exquisite specificity of carefully designed primers. Here I describe the detection of mRNAs for VEGF isoforms in mouse, rat and zebrafish. The protocol may be adapted for detection of any mRNAs by designing primers specific to the genes of interest.
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