聚对苯二甲酸乙二醇酯
下调和上调
纳米颗粒
体外
内吞作用
活性氧
细胞
巨噬细胞
细胞培养
聚乙烯
材料科学
原材料
细胞生物学
化学
纳米技术
生物化学
生物
复合材料
基因
有机化学
遗传学
作者
J. Cristóbal Aguilar-Guzmán,Katarzyna Bejtka,Marco Fontana,Éugenia Valsami-Jones,Anaid Meza Villezcas,Rafael Vázquez-Duhalt,Ana G. Rodríguez-Hernández
标识
DOI:10.1186/s43591-022-00027-1
摘要
Abstract Plastic pollution is a major environmental concern due to its pervasiveness which continues to increase year on year, as a result of a continuing acceleration in global plastic production and use. Polyethylene terephthalate (PET) is among the most produced plastics, commonly used as food and beverage containers. Once released in the environment, the degradation of plastic materials produces micro-and nano-plastics, with a particular concern about potential toxicological effects if they cross epithelial barriers via inhalation or ingestion. In this work, the effect of PET nanoparticles (PET-NPs) (≤ 250 d.nm) was assayed on mouse macrophages cell line (RAW 264.7) in in vitro experiments. Results showed that PET nanoparticles were easily internalized by the cells, 15 μg/mL of nanoparticles concentration had exhibited effects in cell proliferation and a slightly increased production of Reactive Oxygen Species (ROS), which seems to trigger cell response as foreign particles related to upregulation of PCDH12, IGH-V10, ROBO1 genes, and cell maintenance functions, related to FTSJ2 gene upregulation. Thus, the RAW 264.7 results showed here are useful towards for a preliminary and understanding of the potentially toxic effects related to PET nanoparticles and complementary to other in vitro assays, as the first step into the development of the risk assessment framework.
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