血小板
炎症
生物
肿瘤坏死因子α
内分泌学
单核细胞
免疫印迹
内科学
脂多糖
血小板因子4
载脂蛋白B
促炎细胞因子
脂质代谢
油红O
血小板活化
H&E染色
白细胞介素
载脂蛋白E
免疫学
细胞因子
医学
脂肪组织
免疫组织化学
胆固醇
生物化学
疾病
基因
脂肪生成
作者
Xiaowei Liu,Yuanyuan Bao,Lin Zhou,Lijiang Tang,Ping Mao
出处
期刊:Cell Cycle
[Informa]
日期:2022-02-25
卷期号:: 1-11
标识
DOI:10.1080/15384101.2022.2044703
摘要
Platelets can protect from lipopolysaccharide-induced septic shock by inhibiting inflammation, but it is unknown whether platelets have an anti-atherosclerotic effect. The aim of this study was to investigate the effect of platelet transfusion on atherosclerosis (AS) in a mouse model of AS. Apolipoprotein E deficiency (ApoE−/−) mice were fed with a high-fat diet (HFD) for 8 weeks to establish a mouse model of AS. Mice weekly underwent bi-weekly injection with or without platelets during AS induction (HFD+platelet). Hematoxylin–eosin (H&E), Oil Red O, and Sudan IV stainings were used to assess pathological and morphological changes in the aortic tissue. Lipid levels, and liver and kidney function were examined using an automatic biochemical analyzer. Immune histochemical assays were used to detect the infiltration and distribution of inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), IL-6, and monocyte chemotactic protein-1 (MCP-1) in the aortic arch. Western blot and enzyme-linked immunosorbent assay (ELISA) were used to examine the expression levels of TNF-α, IL-1β, IL-6, and MCP-1 in the aorta or the peripheral blood, respectively. Compared with the HFD group, AS pathological lesions from the aortic arch in the HFD+platelet group were significantly smaller and alterations in the lipid metabolism were also less pronounced. Furthermore, TNF-α, IL-1β, IL-6, and MCP-1 levels were all significantly reduced in mice that received platelet injection. Platelets transfusion can effectively ameliorate lipid metabolism, suppress the inflammatory response in the vascular wall, and inhibit the development of AS in mice.
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