High speed autofluorescence imaging of the effects of short pulses of infrared light

Imaging the spatial and temporal effects of millisecond duration pulses of infrared light on neurons requires image frame rates approaching 1000+ Hz to capture neural activity. Autofluorescence imaging of the metabolic coenzymes reduced nicotinamide adenine dinucleotide and flavin adenine dinucleotide provides information about cellular metabolism and can be a surrogate measurement of neural activity. Here, we are combining fast fluorescence microscopy techniques with modeling and machine learning to image autofluorescence dynamics in cells following exposure to infrared light.