自愈水凝胶
化学
生物相容性
生物物理学
弹性蛋白
钙调蛋白
低临界溶液温度
药物输送
流变学
共聚物
乙二醇
高分子化学
材料科学
钙
聚合物
有机化学
生物
医学
病理
复合材料
作者
Jae Sang Lee,Min Jeong Kang,Jae Hee Lee,Dong Woo Lim
出处
期刊:Biomacromolecules
[American Chemical Society]
日期:2022-04-12
卷期号:23 (5): 2051-2063
被引量:9
标识
DOI:10.1021/acs.biomac.2c00053
摘要
A variety of block copolypeptides with stimuli responsiveness have been of growing interest for dynamic self-assembly. Here, multistimuli-responsive triblock copolypeptides composed of thermosensitive elastin-based polypeptides (EBP) and ligand-responsive calmodulin (CalM) were genetically engineered, over-expressed, and nonchromatographically purified by inverse transition cycling. Diluted EBP-CalM-EBP (ECE) triblock copolypeptides under physiological conditions self-assembled into vesicles at the nanoscale by temperature-triggered aggregation of the EBP block with lower critical solution temperature behaviors. Furthermore, concentrated ECE triblock copolypeptides under identical conditions exhibited thermally induced gelation, resulting in physically crosslinked hydrogels. They showed controlled rheological and mechanical properties depending on the conformational change of the CalM middle block induced by binding either Ca2+ or Ca2+ and trifluoperazines (TFPs) as ligands. In addition, both Ca2+-free and Ca2+-bound ECE triblock copolypeptide hydrogels exhibited biocompatibility, while those bound to both Ca2+ and TFPs showed severe cytotoxicity because of controlled TFP release of the CalM blocks. The ECE triblock hydrogels with stimuli responsiveness would be useful as injectable drug delivery depots for biomedical applications.
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