适体
指数富集配体系统进化
寡核苷酸
核酸
计算生物学
SELEX适体技术
生物
定向分子进化
化学
DNA
生物化学
核糖核酸
分子生物学
定向进化
基因
突变体
作者
Ricardo Oliveira,Eva Pinho,Ana Luísa Sousa,Jeffrey J. DeStefano,Nuno F. Azevedo,Carina Almeida
标识
DOI:10.1016/j.tibtech.2021.09.011
摘要
Aptamers are structural single-stranded oligonucleotides generated in vitro to bind to a specific target molecule. Aptamers' versatility can be enhanced with nucleic acid mimics (NAMs) during or after a selection process, also known as systematic evolution of ligands by exponential enrichment (SELEX). We address advantages and limitations of the technologies used to generate NAM aptamers, especially the applicability of existing engineered polymerases to replicate NAMs and methodologies to improve aptamers after SELEX. We also discuss the limitations of existing methods for sequencing NAM sequences and bioinformatic tools to predict NAM aptamer structures. As a conclusion, we suggest that NAM aptamers might successfully compete with molecular tools based on proteins such as antibodies for future application.
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