Cellular biogenesis of metal nanoparticles by water velvet ( Azolla pinnata ): different fates of the uptake Fe 3+ and Ni 2+ to transform into nanoparticles

Plants can produce cellular metal nanoparticles (NPs) from the uptake of metal ions, but the mechanism remains unclear. This work reported the new insight into different fates of iron (Fe) and nickel (Ni) ions to transform into the metal NPs in Azolla pinnata roots. After exposing to ferric nitrate, nickel nitrate, and a combination of both for 12 h, the energy dispersive X-ray fluorescence analysis indicated the efficient uptakes of both metal ions in the roots and their transports into the shoots. Transmission electron microscope images revealed the accumulation of spherical FeNPs, but not NiNPs, near the cell wall and cell membrane, and inside vacuoles and multivesicular bodies in cortical and vascular cells at the root tips. The energy dispersive X-ray analysis suggested that the formation of metal NPs depended on the sufficient concentration of metal ions localized in the roots. FeNPs were identified to ɑ-Fe2O3 and Fe3O4 by selected area electron diffraction analysis. The formation of FeNPs might involve the increase of superoxide dismutase activity. This work is the first report about the cellular biogenesis of metal NPs in plant roots that likely depends on cellular metal content and involves the reducing activity of antioxidant enzymes.