环介导等温扩增
宫颈癌
数字化
数字聚合酶链反应
基因分型
化学
金标准(测试)
癌症
聚合酶链反应
计算机科学
内科学
DNA
医学
基因型
基因
生物化学
计算机视觉
作者
Jiasi Wang,Jeannette P. Staheli,Andrew Wu,Jason E. Kreutz,Qiongzheng Hu,Jingang Wang,Thomas Schneider,Bryant S. Fujimoto,Yuling Qin,Gloria S. Yen,Bob Weng,Kara Shibley,Halia Haynes,Rachel L. Winer,Qinghua Feng,Daniel T. Chiu
标识
DOI:10.1021/acs.analchem.0c04973
摘要
Cervical cancer is the fourth-leading cause of cancer deaths among women worldwide and most cases occur in developing countries. Detection of high-risk (HR) HPV, the etiologic agent of cervical cancer, is a primary screening method for cervical cancer. However, the current gold standard for HPV detection, real-time PCR, is expensive, time-consuming, and instrumentation-intensive. A rapid, low-cost HPV detection method is needed for cervical cancer screening in low-resource settings. We previously developed a digital loop-mediated isothermal amplification (dLAMP) assay for rapid, quantitative detection of nucleic acids without the need for thermocycling. This assay employs a microfluidic self-digitization chip to automatically digitize a sample into an array of nanoliter wells in a simple assay format. Here we evaluate the dLAMP assay and self-digitization chip for detection of the commonly tested 14 high-risk HPVs in clinical samples. The dLAMP platform provided reliable genotyping and quantitative detection of the 14 high-risk HPVs with high sensitivity, demonstrating its potential for simple, rapid, and low-cost diagnosis of HPV infection.
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