[The Content Determination of Biotransformation of Rb1 in the Total Saponins of Panax notoginseng by A Plant Endophyte Coniochaeta sp.].

Objective: To investigate the specific biotransformation product of ginsenoside Rb1 of Panax notoginseng saponins( PNS) by an individual plant endophyte. Methods: The endophytes of an invasive plant were selected as the screening targets of active conversion strains. The chromatography and high performance liquid chromatography were used to detemine the metabolite. Results: Totally, an active strain of conversion of ginsenoside Rb1 were achieved. The strain can specifically convert ginsenoside Rb1 of PNS to ginsenoside Rd and rare saponin ginsenoside C-K,with the conversion rate of 11. 62% of ginsenoside C-K by fermentation for 12 d. Conclusion: This transformation can obviously increase the content of minor ginsenoside C-K in PNS,and it is expected to be a new way to obtain the active saponin ginsenoside C-K in quantity.