Scanning electron microscopy of the liver cell cytoskeleton

Rat livers were perfused with 0.5% Triton X–100 for 30 to 60 min and studied by scanning electron microscope. Three–dimensional filamentous networks were visualized in the cytoplasm of hepatocytes in situ. Branching and end–to–side contacts of intermediate filaments, and intermediate filaments which were connected with microtubules and microfilaments were noted. Numerous filaments were observed in the perinuclear region and at the cell border. Filaments were attached to polyribosomes and nuclei. No differences were observed in the cytoplasmic cytoskeleton after treatment of the sample at 4°C. These results support the concept that intermediate filaments mechanically integrate the cytoplasmic space.