西塔
锌指
发起人
抄写(语言学)
生物
基因
主要组织相容性复合体
转录因子
细胞生物学
MHC I级
遗传学
MHC II级
基因表达
语言学
哲学
作者
Wafa Y. Al-Kandari,Rupa Koneni,Vandana Navalgund,Anastasiia Aleksandrova,Srikarthika Jambunathan,Joseph D. Fontes
标识
DOI:10.1016/j.jmb.2007.04.033
摘要
The transcription of major histocompatibility complex class II (MHC II) genes depends critically upon the activity of the class II trans-activator (CIITA) protein. We previously described a novel CIITA-binding protein named zinc finger X-linked duplicated family member C (ZXDC) that contributes to the activity of CIITA and the transcription of MHC II genes. Here, we examined the contribution of a closely related family member of ZXDC, the ZXDA protein, to MHC II gene transcription. ZXDA has a domain organization similar to ZXDC, containing ten zinc fingers and a transcriptional activation domain. Knockdown and overexpression of ZXDA demonstrated its importance in the transcriptional activation of MHC II genes. We found that ZXDA and ZXDC can self-associate, and also form a complex with each other. The regions of the two proteins that contain zinc fingers mediate these interactions. Importantly, we found that the ZXDA-ZXDC complex interacts with CIITA, rather than either protein alone. Given our additional finding that ZXDC is present at MHC II promoters in HeLa cells, prior to and after treatment with IFN-γ, it appears that ZXDA and ZXDC form an important regulatory complex for MHC II gene transcription.
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