糖原
星形胶质细胞
脱氧葡萄糖
糖原合酶
生物化学
生物
甘露糖
化学
内科学
内分泌学
中枢神经系统
医学
作者
Ralf Dringen,Rolf Gebhardt,Bernd Hamprecht
出处
期刊:Brain Research
[Elsevier]
日期:1993-10-01
卷期号:623 (2): 208-214
被引量:497
标识
DOI:10.1016/0006-8993(93)91429-v
摘要
In order to contribute to the elucidation of the function of astrocyte glycogen in brain, studies on the fate of the glucosyl residues of glycogen were carried out on astroglia-rich primary cultures derived from the brains of newborn rats. On glucose deprivation astroglial cells rapidly deplete their glycogen. In contrast to the situation with hepatocytes, only lactate, but not glucose, is detectable in the medium surrounding the astroglial cells. Besides glucose, astroglial cultures can also use mannose as a substrate for the synthesis of glycogen and the generation of lactate. Although mannose-fed astroglial cells contain glucose-6-phosphate, they do not release a measurable amount of glucose into the culture medium. Instead of glucose the astroglial cells release high amounts of lactate into the culture medium. Gluconolactone or 2-deoxyglucose which prevent glycogen breakdown in astroglial cells after glucose deprivation, allow to discriminate between lactate generated from glycogen and lactate from other sources. The amount of lactate found in the medium in the absence of gluconolactone (or 2-deoxyglucose) exceeds the amount found in the presence of either compound by the lactate equivalents calculated to be contained in the cellular glycogen. In conclusion, glycogen in astrocytes can be considered as a store for lactate rather than for glucose.
科研通智能强力驱动
Strongly Powered by AbleSci AI