核酸
聚合酶链反应
实时聚合酶链反应
计算生物学
DNA
核酸定量
核糖核酸
分子生物学
污染
化学
生物
生物化学
基因
生态学
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2007-07-01
卷期号:2007 (7): pdb.top14-pdb.top14
被引量:21
摘要
INTRODUCTIONDevelopment of the polymerase chain reaction (PCR) as a basic component of the molecular biology laboratory has occurred very rapidly from its inception in 1985. As PCR became more widely used, scientists rapidly learned more about it and, as a result, learned that PCR had its strong points and its deficiencies. Very quickly, PCR demonstrated its power to amplify very small amounts (e.g., a single copy) of template nucleic acid and to amplify different nucleic acids (e.g., DNA and RNA). At the same time, laboratory personnel learned that this biochemical reaction had a unique deficiency, namely, a strong susceptibility to contamination from its own product. This article is devoted to establishing a PCR laboratory whose operations will give reliable and contamination-free results.
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