Protective effects of carvedilol on rats cardiomyocytes exposed to adriamycin

Objective To study the protective effects of carvedilol on rats cardiomyocytes exposed to adriamycin.Methods SD rats were randomly divided into four groups:control group,adriamycin group,adriamycin+carvedilol group,carvedilol group.The models of myocardium toxicity were prepared with intraperitoneal injection of adriamycin(total 15 mg/kg,injection by 6 times);Carve- dilol was applied intraperitoneally followed adriamycin administration with a dose of(total 2 mg/kg,injection by 6 times);the equiva- lent volume of normal saline was used in control group.The heart tissues were obtained at the 21st day after the first time drug adminis- tration.The Western blot were performed to assess the expression alternation of bcl-2,bax,TNF-αand TNF-αR1 in protein levels. Results①The expression of TNF-αshowed clearly in adriamycin group and obscurly or dispeared in,adriamycin+carvedilol group, no sign in carvedilol group and control group.②The expression of bcl-2 showed clearly in carvedilol group and obscurly or dispeared in,adriamycin+carvedilol group,no sign in adriamycin group and control group.Conclusions Carvedilol may promote the expression of bcl-2 and inhibit the expression of TNF-α,but there is no clear influence to bax and TNF-αR1 by carvedilol.Tthese data may provide useful clues to elucidating the protective mechanism of carvedilol in protein levels.