Comparative study of transfection of siRNA to mosquito cells by three different methods of transfection

Objective To compare the advantages and disadvantages of three different transfection reagents for mediated transfection of siRNA to mosquito cells and choose the most suitable for cell viability assay after transfection.Methods The specific siRNA was transfected into mosquito cells using FuGENEHD,X-tremeGENE,and siPORT Amine transfection reagents.Quantitative Real-Time PCR was performed to access gene expression 48 hr after transfection.The transfection efficiency,cytotoxicity,and use of different reagents were compared.Results Transfection efficiency was 70% for FuGENEHD,85% for X-tremeGENE,and 29% for siPORT Amine.FuGENEHD and siPORT Amine had lower cytotoxicity than did X-tremeGENE.During transfection,FuGENEHD in the culture medium had to be replaced once,X-tremeGENE had to be replaced twice,and siPORT did not have to be replaced.Conclusion The efficiency of transfection reagents significantly affected results.FuGENEHD has moderate transfection efficiency,low cytotoxicity,and was only replaced once during transfection.X-tremeGENE has high transfection efficiency but high cytotoxicity and was replaced twice during transfection.siPORTTM Amine has low cytotoxicity and was not replaced but has low transfection efficiency.