In recent years,a great number of yellow catfish (Pelteobagrus fulvidraco)were sick and finally died,in the fishery ponds of Jiangsu province,with the distinctness symptom of belly tumidity,blood in the liver and body surface,no food intake,and soliquoid.In this paper,a dominant bacterial was purified from the ascites,liver,gill and canker skin of the sick yellow fish from several farms.With the help of light microscope,TEM,biochemical characteristics of the bacterial,and the molecular systematic (phylogenetic tree based on 16S rDNA sequences),the bacterial was identified.It was concluded that the bacterial isolated from the sick yellow fish above,was Edwardsiella tarda,which induced the serious Hemorrhagic Sapticemia disease in the fishery ponds in Jiangsu before,with the homology of 99%,and together with Edwardsiella tarda in phylogenetic tree,what's more,with the same biochemical characteristics as Edwardsiella tarda.It was the first report about the prevalence of Edwardsiella tarda in yellow catfish.