Changes of expression of protein and mRNA of synaptophysin and dynamin I in hippocampal of rat with vascular dementia

Objective To explose the changes of expression of protein and mRNA of synaptophysin and dynamin Ⅰ in hippocampal of rat with vascular dementia( VaD). Methods VaD rat models were established by employing improved method of Pulsinelli s four-vessel occlusion. Abilities of learning and memorize of rats were tested by using the Morris water maze. The expressions of synaptophysin and dynamin Ⅰ at mRNA and protein levels were detected by Real time-PCR and Western blotting respectively. The results were compared with sham operation group. Results The escaping latency and the time for passing through target for the first time in VaD group were significantly prolonged than those in sham operation group( all P 0. 01),and the number of crossing the platform was significantly decreased than that in sham operation group( P 0. 05). The expressions of synaptophysin and dynamin Ⅰ at mRNA and protein levels in VaD group were significantly decreased than those in sham operation group( P 0. 05-0. 01). Conclusion Cerebral ischemia can lead to the expressions of synaptophysin and dynamin Ⅰ at mRNA and protein levels decreased,those may related to impairment of the cognition function in VaD.