Objective:To introduce the recent progress on the structural features of Ribosomal DNA internal transcribed spacer sequence and application in parasitology.Methods:Relative literatures were summarized and analyzed.Results:Ribosomal DNA internal transcribed spacer 1and internal transcribed spacer 2 sequence provided very valuable genetic markers in identification of parasites.Conclusion:Ribosomal DNA internal transcribed spacer evolutionary faster than other regions.It had high variability and a lot of genetic information could be acquired.So Ribosomal DNA internal transcribed spacer has become a effective means in the classification identification in parasites species and subspecies.