Pharmacokinetic characteristics and correlation analysis of preparations of salidroside from fructus ligustri lucidi and two pure bioactive components in rat model of migraine

objective To obtain the optimum medicinal system and its composition form,by the way of comparation of pharmacokinetic characteristics of different medicinal forms of salidroside and tyrosol in the rat model of migraine. Methods The plasma concentrations of salidroside and tyrosol after oral administration in rat model were measured by UPLC-PDA, by which the data of pharmacokinetic characteristics were obtained. Then the characteristics of different forms of salidrosides and tyrosol were compared by correlation analysis( PK-DI). Results Both salidroside and tyrosol were detected in preparations group and mixed group( pure salidroside mixed with pure tyrosol). Only salidroside or tyrosol was detected in pure salidroside group and high-dose pure tyrosol group respectively. While nothing was found in low-dose pure tyrosol group whose concentration was equivalent to preparations of salidroside. There was no difference in bioavailability of salidroside showed in preparation group,mixed group and pure salidroside group,on the contrary,those of tyrosol showed significant difference in preparation group,mixed group,low-dose tyrosol in ascending order. Conclusion Salidroside was retained unchanged in migraine model rat after oral administration,however,for tyrosol,a substantial increase of maximum plasma concentration and changed pharmacokinetics parameters showed under the condition of salidroside coexisted. The best medicinal system in treating migraine was found and supported by synergistic effects of salidroside and tyrosol,by which provide techniques for medicinal research and development.