According to the biological non smooth theory, the design of non smooth surface morphology, mechanical and laser processing method, the formation of similar organisms form non smooth surface on the surface of 45 steel; wear test, laser machining and smooth specimen samples and samples in the bionic micro friction wear test machine, the test results of qualitative and quantitative analysis non smooth surface in the mechanical parts wear resistance of the role; the smooth, bionic coupling, mechanical processing of wear test, found that wear resistance of specimens with optimal laser bionic coupling surface, followed by machining the bionic sample, unprocessed smooth surface wear resistance of the sample is the worst.