磷酸烯醇式丙酮酸羧化酶
转化酶
蔗糖磷酸合酶
蔗糖
APX公司
光合作用
耐旱性
蔗糖合成酶
糖
景天酸代谢
转基因水稻
超氧化物歧化酶
植物
过氧化物酶
生物
化学
园艺
丙酮酸羧化酶
生物化学
酶
转基因
转基因作物
基因
作者
Chen Zhang,Xia Li,Ya-Fei He,Jinfei Zhang,Ting Yan,Xiaolong Liu
标识
DOI:10.1016/j.plaphy.2017.03.019
摘要
We compared the drought tolerance of wild-type (WT) and transgenic rice plants (PC) over-expressing the maize C4PEPC gene, which encodes phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) gene, and evaluated the roles of saccharide and sugar-related enzymes in the drought response. Pot-grown seedlings were subjected to real drought conditions outdoors, and the yield components were compared between PC and untransformed wild-type (WT) plants. The stable yield from PC plants was associated with higher net photosynthetic rate under the real drought treatment. The physiological characters of WT and PC seedlings under a simulated drought treatment (25% (w/v) polyethylene glycol-6000 for 3 h; PEG 6000 treatment) were analyzed in detail for the early response of drought. The relative water content was higher in PC than in WT, and PEPC activity and the C4-PEPC transcript level in PC were elevated under the simulated drought conditions. The endogenous saccharide responses also differed between PC and WT under simulated drought stress. The higher sugar decomposition rate in PC than in WT under drought analog stress was related to the increased activities of sucrose phosphate synthase, sucrose synthase, acid invertase, and neutral invertase, increased transcript levels of VIN1, CIN1, NIN1, SUT2, SUT4, and SUT5, and increased activities of superoxide dismutase and peroxidase in the leaves. The greater antioxidant defense capacity of PC and its relationship with saccharide metabolism was one of the reasons for the improved drought tolerance. In conclusion, PEPC effectively alleviated oxidative damage and enhanced the drought tolerance in rice plants, which were more related to the increase of the endogenous saccharide decomposition. These findings show that components of C4 photosynthesis can be used to increase the yield of rice under drought conditions.
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