Fluorescence detection platform of metal-organic frameworks for biomarkers

Sensitive and selective detection of biomarkers is crucial in the study and early diagnosis of diseases. With the continuous development of biosensing technologies, fluorescent biosensors based on metal-organic frameworks have attracted increasing attention in the field of biomarker detection due to the combination of the advantages of MOFs, such as high specific surface area, large porosity, and structure with tunable functionality and the technical simplicity, sensitivity and efficiency and good applicability of fluorescent detection techniques. Therefore, researchers must understand the fluorescence response mechanism of such fluorescent biosensors and their specific applications in this field. Of all biomarkers applicable to such sensors, the chemical essence of nucleic acids, proteins, amino acids, dopamine, and other small molecules account for about a quarter of the total number of studies. This review systematically elaborates on four fluorescence response mechanisms: metal-centered emission (MC), ligand-centered emission (LC), charge transfer (CT), and guest-induced luminescence change (GI), presenting their applications in the detection of nucleic acids, proteins, amino acids, dopamine, and other small molecule biomarkers. In addition, the current challenges of MOFs-based fluorescent biosensors are also discussed, and their further development prospects are concerned.