GSDMD- and PAD4-Independent NET Formation Mediates Vascular Occlusions in Septicemia

Introduction Generation of Neutrophil Extracellular Traps (NETs), also termed NETosis, contribute to host defense; however, excessive NETosis leads to hyperinflammatory, autoimmune and thrombotic disease states. Histone citrullination by peptidyl arginine deiminase 4 (PAD4), and pore formation in the plasma membrane by gasdermin D (GSDMD) are believed to be involved in the formation and release of NETs [1] [2]. In this study, we aimed to study the role of GSDMD and PAD4 in NET formation in experimental models of septicemia and sterile neutrophilia.