纳米载体
细胞毒性
双金属片
化学
氟尿嘧啶
壳聚糖
核化学
共轭体系
胰腺癌
结合
纳米颗粒
材料科学
纳米技术
癌症
生物化学
金属
体外
有机化学
医学
内科学
数学
数学分析
聚合物
作者
Kandasamy Saravanakumar,Anbazhagan Sathiyaseelan,Panchanathan Manivasagan,Xin Zhang,Myeong Seon Jeong,Eue‐Soon Jang,Myeong‐Hyeon Wang
标识
DOI:10.1016/j.ijbiomac.2023.129165
摘要
This work aimed to prepare multifunctional aptamer-conjugated, photothermally responsive 5-fluorouracil (5fu)-loaded chitosan-bimetallic (Au/Pd) nanoparticles (APT-CS-5fu-Au/Pd NPs) for improved cytotoxicity in two cancer cell lines (PANC-1 and MDA-MD 231). The CS-5fu-Au/Pd NPs were polydispersed with a size of 34.43 ± 1.59 nm. FTIR analysis indicated the presence of CS, 5fu in CS-5fu-Au/Pd NPs. The 2 theta degrees in CS-5fu-Au/Pd NPs accounted for CS and Au/Pd. Additionally, AGE revealed the conjugation of APT in CS-5fu-Au/Pd NPs. The APT-CS-5fu-Au/Pd NPs (180 μg/mL) with NIR treatment increased the temperature to >50 °C. The optimized 5fu input was 0.75 % in CS-5fu-Au/Pd NPs, exhibiting a hydrodynamic size of 141.63 ± 22.86 nm, DEE of 64.2 ± 3.77 %, and DLE of 11.1 ± 0.65 %. A higher level of 5fu release (69.8 ± 2.78 %) was observed under pH 5.4 at 74 h. In conclusion, APT-NIR-CS-5fu-Au/Pd NPs did not cause toxicity to RBC and Egg CAM, but increased cytotoxicity in MDA-MB 231 and PANC-1 cells by triggering oxidative stress-mediated cell death.
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