外周血单个核细胞
免疫系统
免疫学
体外
免疫荧光
疾病
生物
巨噬细胞
医学
病理
抗体
生物化学
作者
Mary Dover,Michael M. Madani,Milan Fiala,Ketema N. Paul
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 123-136
标识
DOI:10.1007/978-1-0716-3754-8_9
摘要
The role of immune system in the progression of neurodegenerative diseases has been studied for decades in animal models. However, invasive studies in human subjects remain controversial due to the heterogeneity of the presentation of different diagnostic categories at different stages of the disease. Peripheral blood mononuclear cells (PBMCs) contain immune cells including dendritic cells (DCs), monocytes, macrophages, and T lymphocytes. Isolating PBMCs from whole blood samples collected from patients provides a minimally invasive method for analyzing the immune system's function in patients with neurodegenerative diseases. By isolating single cell types from patients' peripheral blood, in vitro analyses can be conducted including RNA sequencing, immunofluorescence, and phagocytic analysis. In this chapter, we discuss PBMC separation and isolation of macrophages in pure culture in vitro. We also outline methods for performing RNA-seq on cultured macrophages and other techniques for investigating the role of macrophages in neurodegenerative disease pathophysiology.
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