米诺环素
四环素
抗生素
人血浆
四环素类抗生素
化学
药理学
色谱法
微生物学
医学
生物
生物化学
作者
Dinesh S. Patel,Naveen Sharma,Madhabhai M. Patel,Bhavin Patel,Pranav S. Shrivastav,Mallika Sanyal
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2011-10-01
卷期号:3 (19): 2177-2194
被引量:10
摘要
A simple, selective and sensitive LC-MS/MS assay has been developed for the determination of minocycline in human plasma. Plasma samples were prepared by protein precipitation, followed by chromatographic separation on a HyPURITY™ C8 (100 mm × 4.6 mm, 5 µm) column under isocratic conditions. The LOD and LLOQ of the method were 0.50 and 20.0 ng/ml, respectively. The intra-batch and inter-batch precision (% coefficient of variation) across quality control levels was less than 8.0%. For a set of incurred samples the percentage change in concentration was within ± 9.0%. The method was successfully applied to a bioequivalence study of 135 mg minocycline tablet formulation in 14 healthy Indian males. The reproducibility in the measurement of study data was demonstrated by incurred sample reanalysis.
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