Continuous Synthesis of Polydatin by Dual Enzyme Coupling Reaction and Its Kinetic Study in Microreactors

Resveratrol is a promising ingredient in functional food products, but its low bioavailability and solubility hinder its application. Polydatin, a 3-OH glycosylation of resveratrol, has been shown to exhibit enhanced bioavailability and more favorable physicochemical properties. In this work, a continuous-flow microreactor was developed to synthesize polydatin using resveratrol and a mutant glycosyltransferase (UGTBS) as the substrate and biocatalyst, respectively. All reaction orders were determined to elucidate the possible reaction mechanism. To further reduce the process costs, a dual enzyme coupling reaction system was developed to enable the in situ regeneration of UDP-Glc.