Sustainable supply of wilforic acid C via decompartmentalization of peroxisomal acetyl-CoA and systematic engineering in yeast

Decompartmentalization of peroxisomal acetyl-CoA in engineered S. cerevisiae enables 584.78 mg L −1 wilforic acid C, providing a sustainable platform for celastrol precursors and a generalizable strategy for the biosynthesis of acetyl-CoA products.