转移RNA
化学
核糖体
翻译(生物学)
氨基酸
大肠杆菌
肽
蛋白质生物合成
核糖体RNA
生物化学
肽合成
翻译系统
立体化学
信使核糖核酸
核糖核酸
基因
体外
作者
Zhongping Tan,Anthony Forster,Stephen C. Blacklow,Virginia W. Cornish
摘要
Using a pure Escherichia coli translation system, we tested the intrinsic specificity of the protein biosynthetic machinery by determining the relative yields of peptide synthesis for incorporation of a series of acyl-%@mt;sys@%tRNA%@sx@%GAC%@be@%AsnB%@sxx@%%@mx@% 's with varied backbone structures at the sense codon GUU (Val). The results showed that different amino acids on the same tRNA adaptor give significantly different peptide yields and the potential for cross-talk between the amino acid and tRNA body/anticodon in aa-tRNA decoding by the ribosome. They further support the substrate plasticity of the ribosomal biosynthetic machinery and provide immediate candidates for ribosomally encoded polymer synthesis.
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