化学
壳聚糖
生物传感器
复合数
基质(化学分析)
结直肠癌
纳米技术
电化学
多孔性
DNA
序列(生物学)
纳米-
化学工程
组合化学
色谱法
癌症
电极
生物化学
有机化学
复合材料
物理化学
内科学
材料科学
医学
工程类
作者
Keqin Feng,Yunhui Yang,Zhijie Wang,Jian‐Hui Jiang,Guo‐Li Shen,Ru‐Qin Yu
出处
期刊:Talanta
[Elsevier]
日期:2006-10-15
卷期号:70 (3): 561-565
被引量:153
标识
DOI:10.1016/j.talanta.2006.01.009
摘要
CeO2/Chitosan (CHIT) composite matrix was firstly developed for the single-stranded DNA (ssDNA) probe immobilization and the fabrication of DNA biosensor related to the colorectal cancer gene. Such matrix combined the advantages of CeO2 and chitosan, with good biocompatibility, nontoxicity and excellent electronic conductivity, showing the enhanced loading of ssDNA probe on the surface of electrode. The preparation method is quite simple and inexpensive. The hybridization detection was accomplished by using methylene blue (MB), an electroactive lable, as the indicator. The differential pulse voltammetry (DPV) was employed to record the signal response of MB and determine the amount of colorectal cancer target DNA sequence. The experimental conditions were optimized. The established biosensor has high detection sensitivity, a relatively wide linear range from 1.59 × 10−11 to 1.16 × 10−7 mol L−1 and the ability to discriminate completely complementary target sequence and four-base-mismatched sequence.
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