体内
植入
重组DNA
骨愈合
骨形态发生蛋白
骨形态发生蛋白2
皮下注射
骨重建
骨形成
大肠杆菌
生物医学工程
体外
医学
化学
内科学
生物
解剖
外科
生物化学
生物技术
基因
作者
Ji Hyun Lee,Chang Sung Kim,Kyung Hee Choi,Ui Won Jung,Jin-Mun Yun,Seong Ho Choi,Kyoo-Sung Cho
出处
期刊:Biomaterials
[Elsevier]
日期:2010-05-01
卷期号:31 (13): 3512-3519
被引量:106
标识
DOI:10.1016/j.biomaterials.2010.01.075
摘要
We investigated the ability of recombinant human bone morphogenetic protein-2, produced from Escherichia coli (ErhBMP-2), to form orthotopic and ectopic bone in rat models. BMP-2 was expressed in E. coli and extracted from the inclusion bodies. Critical-sized calvarial defects and subcutaneous pouches were created in rats, and an absorbable collagen sponge (ACS) was loaded with different doses of ErhBMP-2 for implantation. ACS alone and sham surgery controls were also included. Implant sites were evaluated by histological and/or histometric analyses following a 2- or 8-week healing interval. In the calvarial defect model, enhanced bone formation was observed with all doses of ErhBMP-2, while only limited amounts of new bone were found in controls. In the ectopic subcutaneous implant model, bone formation was clearly observed in all animals treated with ErhBMP-2 at 2 weeks. However, at 8 weeks, less new bone formation was detected than at 2 weeks. Nevertheless, the remaining new bone showed an advanced degree of bone remodeling and more maturity than that observed at 2 weeks. These results showed that ErhBMP-2 was osteoinductive under controlled in vivo conditions. Thus, ErhBMP-2 has definite potential as an alternative to rhBMP-2 produced in a eukaryotic system for clinical use.
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