核糖核酸酶
镰刀菌
残留物(化学)
重组DNA
化学
发病机制
生物化学
核糖核酸
作用机理
水解
镰刀菌
生物
基因
体外
遗传学
免疫学
作者
Carlo Caporale,Iris Di Berardino,Luca Leonardi,Laura Bertini,A Cascone,Vincenzo Buonocore,Carla Caruso
出处
期刊:FEBS Letters
[Wiley]
日期:2004-08-28
卷期号:575 (1-3): 71-76
被引量:77
标识
DOI:10.1016/j.febslet.2004.07.091
摘要
We have demonstrated that wheatwin1, a wheat pathogenesis-related protein of class 4 (PR4), has ribonuclease activity. Both native and recombinant proteins hydrolyse RNA from wheat coleoptils and have antifungal activity. Sepharose-bound wheatwin1 is able to interact with either wheat or Fusarium culmorum RNA. 3D modelling studies showed that, like ribonucleases A and T1, the action mechanism should involve two His residues, an Arg residue and an Asp residue.
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