FACILE IMAGE-BASED CELL SORTING USING OPTO- FLUCS (OPTO-FLUIDIC CELL SORTING)

We present a new approach for sorting cells based upon visual information, termed opto-fluidic cell sorting (OPTO-FluCS). This technique uses a combination of hydrodynamic and optical scattering forces to array, image, and then sort many individual cells. Our device combines the strengths of microscopy–imaging in space and time–with the ability to array cell populations and isolate cells of interest in an intuitive, user-friendly fashion. We successfully demonstrate image-based sorting based on fluorescence localization, a sort unavailable with flow-assisted cell sorting.