The effects of graphene on low-temperature anammox process: The insights from short-term tests and long-term operation

Effluent quality deterioration caused by seasonal low temperature is a great challenge to the application of anammox technology. Here, the effects of different graphene materials on anammox process were investigated under both optimal temperature and low-temperature. The batch tests showed that at 30 °C, 300 mg/L of reduced graphene oxide‑sodium alginate gel (RGOSA) had the most significant promoting effect, reaching nitrogen removal efficiency (NRE) and nitrogen removal rate (NRR) of 95 % and 8.88 mgN/L/d, respectively. The changes of EPS secretion patterns and increasing of key enzymes activity might contribute to the enhanced anammox activity. During the long-term operation of anammox reactor, the NRE and NRR of the reactor decreased when the temperature dropped to 15 °C, showing an NRE of 50 %-57 % with the addition of 200 mg/L of reduced graphene oxide (RGO) and 40 %-45 % with the addition of 20 mg/L of RGO. Furthermore, specific anammox activity (SAA) of the RGO200 reactor at 15 °C increased by 57.1 % compared to the UASB reactor without graphene addition. Additionally, 16S rRNA and metagenomic analysis results revealed anammox bacteria Ca. Kuenenia was the dominant bacteria. Moreover, the RGO can significantly increase the relative abundance of N-converting functional genes. This study demonstrates the graphene materials can help anammox process adapting to low temperatures, providing a possible solution for the application of anammox technology.