Polymorphospora lycopeni sp. nov., a lycopene-producing actinomycetes isolated from lakeside soil sample of Baiyangdian

A novel actinomycetes producing lycopene, designated HBU208002 T , was isolated from a lakeside soil sample collected in Baiyangdian, located in Xiong’an New Area of China, and its taxonomic position was investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that the strain HBU208002 T fell within the genus Polymorphospora and was closely related to Polymorphospora rubra JCM 14904 T (99.73% identity). However, the average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH) and average amino acid identity (AAI) values between the strain HBU208002 T and P. rubra JCM 14904 T were 91.78, 44.7 and 91.6%, respectively, which were lower than the ANI (95–96%), DDH (>70%) and AAI (>95%) thresholds of prokaryotic microbial defined species. The predominant fatty acids of the strain HBU208002 T were iso-C 16:0 , C 17:1 ω8c. The menaquinones of the strain HBU208002 T were MK-8(H 8 ) and MK9(H 4 ), while those of P. rubra JCM 14904 T were MK-10(H 6 ), MK-10(H 4 ), MK-9(H 6 ) and MK-9(H 4 ). Meanwhile, some phenotypic characterizations and antibacterial activities distinguished the strain HBU208002 T from P. rubra JCM 14904 T . The strain HBU208002 T exhibited inhibitory effects on Fusarium graminearum , Fusarium verticillioides and Botrytis cinerea , but P. rubra JCM 14904 T had no activity. Therefore, the strain HBU208002 T should be assigned as representing a novel species of the genus Polymorphospora , for which the name Polymorphospora lycopeni was proposed. The type strain is HBU208002 T (=KCTC49833 T = GDMCC4.236 T ).