生物矿化
聚电解质
化学
矿化(土壤科学)
生物物理学
聚乙烯亚胺
高分子
聚合物
矿化组织
化学工程
材料科学
生物化学
有机化学
牙本质
氮气
工程类
生物
转染
基因
复合材料
作者
Yiru Wang,Yizhou Zhang,Zhe Shi,Ye Qiu,Chaoyang Wang,Zhifang Wu,Minjuan Shen,Changyu Shao,Ruikang Tang,Matthias Hannig,Bojie Fu,Zihuai Zhou
标识
DOI:10.1002/adhm.202400102
摘要
Abstract The phosphorylated noncollagenous proteins (NCPs) play a vital role in manipulating biomineralization, while the mechanism of phosphorylation of NCPs in intrafibrillar mineralization of collagen fibril has not been completely deciphered. Poly(vinylphosphonic acid) (PVPA) and sodium trimetaphosphate (STMP) as templating analogues of NCPs induce hierarchical mineralization in cooperation with indispensable sequestration analogues such as polyacrylic acid (PAA) via polymer‐induced liquid‐like precursor (PILP) process. Herein, we propose STMP‐Ca and PVPA‐Ca complexes to achieve rapid intrafibrillar mineralization through polyelectrolyte‐Ca complexes pre‐precursor (PCCP) process. This strategy is further verified effectively for remineralization of demineralized dentin matrix both in vitro and vivo . Although STMP micromolecule fails to stabilize amorphous calcium phosphate (ACP) precursor, STMP‐Ca complexes facilely permeate into intrafibrillar interstices and trigger phase transition of ACP to hydroxyapatite within collagen. In contrast, PVPA‐stabilized ACP precursors lack liquid‐like characteristic and crystallize outside collagen due to rigid conformation of PVPA macromolecule, while PVPA‐Ca complexes infiltrate into partial intrafibrillar intervals under electrostatic attraction and osmotic pressure as evidenced by intuitionistic three‐dimensional stochastic optical reconstruction microscopy (3D‐STORM). The study not only extends the variety and size range of polyelectrolyte for PCCP process but also sheds light on the role of phosphorylation for NCPs in biomineralization. This article is protected by copyright. All rights reserved
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