即时
生物污染
生物正交化学
纳米技术
微流控
材料科学
微气泡
生物物理学
化学
点击化学
化学工程
生物
组合化学
生物化学
医学
工程类
放射科
超声波
膜
食品科学
作者
Yuanhang Xiang,Hui Zhang,Hao Lü,Binqi Wei,Cuiyun Su,Xiaojie Qin,Min Fang,Xinchun Li,Fan Yang
出处
期刊:ACS Nano
[American Chemical Society]
日期:2023-05-05
卷期号:17 (10): 9633-9646
被引量:7
标识
DOI:10.1021/acsnano.3c03194
摘要
Integrating clinical rare cell enrichment, culture, and single-cell phenotypic profiling is currently hampered by the lack of competent technologies, which typically suffer from weak cell-interface collision affinity, strong nonspecific adsorption, and the potential uptake. Here, we report cells-on-a-bubble, a bioinspired, self-powered bioorthogonal microbubble (click bubble) that leverages a clickable antifouling nanointerface and a DNA-assembled sucker-like polyvalent cell surface, to enable instant and suspended isolation of circulating tumor cells (CTCs) within minutes. Using this biomimetic engineering strategy, click bubbles achieve a capture efficiency of up to 98%, improved by 20% at 15 times faster over their monovalent counterparts. Further, the buoyancy-activated bubble facilitates self-separation, 3D suspension culture, and in situ phenotyping of the captured single cancer cells. By using a multiantibody design, this fast, affordable micromotor-like click bubble enables suspended enrichment of CTCs in a cohort (n = 42) across three cancer types and treatment response evaluation, signifying its great potential to enable single-cell analysis and 3D organoid culture.
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