生物过程
废水
流出物
食品科学
单细胞蛋白
重新使用
制浆造纸工业
糖
酵母
食品加工
生物技术
硫酸铵
生物
化学
环境科学
发酵
环境工程
生物化学
色谱法
工程类
生态学
古生物学
作者
Omar Pillaca‐Pullo,André Moreni Lopes,Liz M. I Rodriguez‐Portilla,Waldir D. Estela‐Escalante
摘要
Abstract Background Coffee production is an important agro‐industrial activity in several countries worldwide. However, during wet processing of coffee beans, substantial quantities of wastewater effluents are generated and discharged into nearby water systems, negatively impacting ecosystems. A compelling biotechnological alternative involves the use of nutrients contained in wastewater ( e.g. , sugars, proteins, and salts) to produce value‐added molecules–such as single‐cell protein (SCP) using the yeast Candida sorboxylosa –with high potential for use as animal feed . Results Yeast isolated from the feces of ring‐tailed coatis showed a positive assimilation of reducing sugars (glucose, mannose, and fructose). Efficient reuse of coffee wastewater (CWW) for SCP production was achieved via the statistical design of experiments (DoE) technique. The bioprocess was standardized in a medium culture composed of 87.5% (v/v) CWW, and of 1.38 and 7.24 g/L yeast extract (YE) and ammonium sulfate [(NH 4 ) 2 SO 4 ], respectively, resulting in a high SCP yield of approximately 38%. Conclusion The reuse of CWW shows potential as a low‐cost component for SCP production that can ultimately reduce the environmental impact of wastewater effluent by circumventing its release into river systems. The bioprocess developed in this study presented certain advantages, including high volumes of CWW with only YE and (NH 4 ) 2 SO 4 supplementation, and without requiring additional components to correct the pH of the medium. Therefore, it is highly feasible to apply this bioprocess in close proximity to coffee‐processing agrobusinesses, thus generating an alternative source of income for Peruvian coffee‐producing communities. © 2022 Society of Chemical Industry (SCI).
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