戊二醛
生物相容性
体内
多孔性
生物医学工程
微型多孔材料
肿胀 的
大豆蛋白
材料科学
活力测定
生物降解
伤口愈合
组织工程
化学
化学工程
体外
复合材料
色谱法
外科
食品科学
生物化学
有机化学
生物技术
医学
生物
工程类
作者
Neelima Varshney,Priya Singh,Rohit Rai,Niraj Kumar Vishwakarma,Sanjeev Kumar Mahto
标识
DOI:10.1016/j.ijbiomac.2023.127268
摘要
Soy protein isolate (SPI) has received widespread attention of the biomedical research community primarily due to its good biocompatibility, biodegradability, high availability and low cost. Herein, glutaraldehyde cross-linked microporous sponge-like SPI scaffolds were prepared using the cryogelation technique for tissue engineering applications. The prepared SPI scaffolds possess an interconnected porous structure with approximately 90% porosity and an average pore size in the range of 45-92 μm. The morphology, porosity, swelling capacity and degradation rate of the cryogels were found to be dependent on the concentration of polymer to crosslinking agent. All cryogels were found to be elastic and able to maintain physical integrity even after being compressed to one-fifth of their original length during cyclic compression analysis. These cryogels showed excellent mechanical properties, immediate water-triggered shape restoration and absorption speed. Furthermore, cryogels outperformed cotton and gauze in terms of blood clotting and blood cell adherence. The in vitro and in vivo studies demonstrated the potency of SPI scaffolds for skin tissue engineering applications. Our findings showed that crosslinking with glutaraldehyde had no detrimental effects on cell viability. In addition, an in vivo wound healing study in rats validated them as good potential wound dressing materials.
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