Facile Synthesis of Self‐Targeted Zn2+‐Gallic acid Nanoflowers for Specific Adhesion and Elimination of Gram‐Positive Bacteria

Abstract Transition metal ions are served as disinfectant thousand years ago. However, the in vivo antibacterial application of metal ions is strongly restricted due to its high affinity with proteins and lack of appropriate bacterial targeting method. Herein, for the first time, Zn 2+ ‐gallic acid nanoflowers (ZGNFs) are synthesized by a facile one‐pot method without additional stabilizing agents. ZGNFs are stable in aqueous solution while can be easily decomposed in acidic environments. Besides, ZGNFs can specifically adhere onto Gram‐positive bacteria, which is mediated by the interaction of quinone from ZGNFs and amino groups from teichoic acid of Gram‐positive bacteria. ZGNFs exhibit high bactericidal effect toward various Gram‐positive bacteria in multiple environments, which can be ascribed to the in situ Zn 2+ release on bacterial surface. Transcriptome studies reveal that ZGNFs can disorder basic metabolic processes of Methicillin‐resistant Staphylococcus aureus (MRSA). Moreover, in a MRSA‐induced keratitis model, ZGNFs exhibit long‐term retention in the infected corneal site and prominent MRSA elimination efficacy due to the self‐targeting ability. This research not only reports an innovative method to prepare metal‐polyphenol nanoparticles, but also provides a novel nanoplatform for targeted delivery of Zn 2+ in combating Gram‐positive bacterial infections.