Development of gelatin-coated nanoliposomes loaded with β-cyclodextrin/vitamin D3 inclusion complex for nutritional therapy

The aim of this study was to synthesize of β-cyclodextrin/Vitamin D3 (βCD/VitD3) inclusion complex and encapsulation of this complex with gelatin-coated nanoliposomes (NLPs). Fourier transform infrared spectroscopy confirmed the formation of βCD/VitD3 inclusion complex. In the next step, different gelatin concentrations (1, 2, and 4 mg/mL) were used to surface coat the blank NLPs. The concentration of 2 mg/mL of gelatin was chosen as the optimal concentration for coating the complex-loaded NLPs by considering particle size, morphology, and zeta potential. The particle size and zeta potential of the coated complex-loaded NLPs were 117 ± 2.55 nm and 19.8 ± 1.25 mV, respectively. The images taken by transmission electron microscopy confirmed the formation of a biopolymer layer of gelatin around the NLPs' vesicles. The complex encapsulation efficiency inside the NLPs was 81.09%. The βCD/VitD3 complex loaded NLPs and its coated form exhibited a controlled release profile in simulated gastrointestinal condition.