化学
色谱法
激素
串联质谱法
质谱法
液相色谱-质谱法
内生
胆汁酸
生物化学
作者
Yun Li,Dan Zhang,Yan Mo,Teng Zeng,Tongzhi Wu,Lingqiao Liu,Hua Zhang,Chengzhi Chen
标识
DOI:10.1016/j.jpba.2022.115139
摘要
Endogenous steroids, including sex hormones and bile acids, are a group of essential compounds with various biological functions. In this study, we developed an LC-MS method that simultaneously measures 14 sex hormones and metabolites (SH) and 32 bile acids (BA) in rat plasma. Multiple innovative approaches were applied to increase the sensitivity and specificity, including optimization of the mobile phases, gradients, and dynamic multiple reaction monitoring (DMRM) transitions. The method was validated and applied on plasma samples from pregnant rats before and 0.5 h after oral glucose tolerance test (OGTT) at gestational days 0.5 and 18.5. Results showed that the method was applicable, and 9 SH and 30 BA were measurable in the samples. In summary, this method is applicable in studies on SH and BA in rat plasma, and may also be used on other matrix and species.
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