小RNA
细胞外小泡
胞外囊泡
化学
生物分析
DNA
临床诊断
分子生物学
计算生物学
微泡
纳米技术
生物物理学
细胞生物学
基因
生物
生物化学
色谱法
材料科学
医学
临床心理学
作者
Yuanyuan Zhang,Wenbin Li,Tingting Ji,ShiHua Luo,Jiuxiang Qiu,Bo Situ,Bo Li,Xiaohe Zhang,Tiange Zhang,Wen Wang,Yunju Xiao,Lei Zheng,Xiaohui Yan
标识
DOI:10.1186/s12951-022-01700-6
摘要
Abstract The profiling of small extracellular vesicle-associated microRNAs (sEV-miRNAs) plays a vital role in cancer diagnosis and monitoring. However, detecting sEV-miRNAs with low expression in clinical samples remains challenging. Herein, we propose a novel electrochemical biosensor using localized DNA tetrahedron-assisted catalytic hairpin assembly (LDT-CHA) for sEV-miRNA determination. The LDT-CHA contained localized DNA tetrahedrons with CHA substrates, leveraging an efficient localized reaction to enable sensitive and rapid sEV-miRNA measurement. Based on the LDT-CHA, the proposed platform can quantitatively detect sEV-miRNA down to 25 aM in 30 min with outstanding specificity. For accurate diagnosis of gastric cancer patients, a combination of LDT-CHA and a panel of four sEV-miRNAs (sEV-miR-1246, sEV-miR-21, sEV-miR-183-5P, and sEV-miR-142-5P) was employed in a gastric cancer cohort. Compared with diagnosis with single sEV-miRNA, the proposed platform demonstrated a higher accuracy of 88.3% for early gastric tumor diagnoses with higher efficiency (AUC: 0.883) and great potential for treatment monitoring. Thus, this study provides a promising method for the bioanalysis and determination of the clinical applications of LDT-CHA. Graphical Abstract
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